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Encompassing Amino Acids: 1-322
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The assay was performed by UV absorption using recombinant ARG1 with thioarginine substrate (Cayman 80240). The UV absorption signal at 412 nm is proportional to the amount of reaction product produced. Reactions were conducted for 30 min at room temperature. To initiate the reaction various concentrations of ARG1 (0-100 nM final concentration) were added to ARG1 assay buffer containing the thioarginine substrate. Absorption signals were measured using a Tecan Infinite M1000 plate reader.
Description: Human Arginase 1 (ARG1) protein, also known as LCN7 and LIECF3, GenBank Accession No. NM_000045, amino acids 1-322, with a C-terminal His-tag, expressed in an HEK293 cell expression system. MW = 36 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_000045
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C terminal His-Tag
Uniprot: P05089
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Sedbrook, J.C., et al., PNAS. 1999 Feb; 96(3):1140-1145.2.Woll, P.J., et al., PNAS. 1998 Mar; 85(6):1859-1863.
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