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Encompassing Amino Acids: 649-946
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
Description: Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_005923.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_005923
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q99683
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ichijo, H. et al., Science 275: 90-94, 1997.
2. Nishitoh, H. et al., Molec. Cell 2: 389-395, 1998.
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