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Encompassing Amino Acids: 2-403 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using KEMPtide substrate (0.2 mg/ml) and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.
Description: Human Aurora kinase A also known as, AURKA, Serine/Threonine-Protein Kinase Aurora-A, and Breast Tumor-Amplified Kinase, GenBank Accession No. NM_003600, a.a. 2-403 (end) with N-terminal His-tag, expressed in a HEK 293 cell expression system. MW =46 kDa.
Format: Aqueous solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200mM Imidazole, 20% glycerol, and 3 mM DTT.
Genbank: NM_003600
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: O14965
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ouchi, M. et al., J. Biol Chem. 279 (19),19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5(6), 853-864 (2003).
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