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Encompassing Amino Acids: 2-344
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay buffer: 50 mM HEPES,pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mMDTT, 3 uM Na-orthovanadate, 0.5 mMATP, 4 uM aurora B substrate, and 4ug/ml Aurora B kinase.
Description: Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_004217
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: Q96GD4
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Delaval, B. et al., Oncogene 23 (26), 4516-4522 (2004).
2. Yasui, Y. et al., J. Biol. Chem. 279, 12997-13003 (2004).