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Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Assay Conditions: Reaction done similar to CTLA4:B7-1[biotin] assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (mouse CTLA4) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (mouse B7-1[B]) (0-50 ng) added for 2h at RT. Strep-HRP added for 1h at RT.
Description: Mouse secreted B7-1, also known as CD80, GenBank Accession No. NM_009855, a.a 38-246, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. MW=52 kDa. Biotinylation is confirmed to be ≥90%.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_009855
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Fc-Avi-tag
Uniprot: Q00609
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Pentcheva-Hoang, T., et. al. Immunity. 2004 Sep; 21(3): 401-413. 2. Hirano, F., et al., Cancer Research. 2005 Feb; 65(3): 1089-1096.
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