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Encompassing Amino Acids: 33-194
Application: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Description: Human secreted B7-H5 Fc fusion protein, also known as VISTA and VSIR, with C-terminal Avi-Tag. GenBank Accession No. NM_022153, a.a 33-194 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 47 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_022153
Storage Stability: At least 6 months at -80°C. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: Fc Fusion, C-terminal Avi-Tag
Uniprot: Q9H7M9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Zhu, Y., et al. Nature Communications. 2013 Feb 27; 4. 2.Jung, K., et al., Immune Network. 2013, Oct. 13;6:184-193.
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