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Applications: Useful for studying protein binding and screening small molecules.
Description: Human secreted B7-H5, Fc fusion protein, also known as Gi24, Dies1, VISTA, and SISP1, GenBank Accession No. NM_022153, a.a. 33-194 expressed in a HEK293 cell expression system. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_022153
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C
Tags: C-terminal Fc fusion (Human IgG1)
Uniprot: Q9H7M9
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Byrum, J.N., et al., J. Immunol. 2013 Sep 15;191(6):3073-3081.
2. Zhu, Y., et al., Nat Commu. 2013;4:2043.
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