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Encompassing Amino Acids: 134 - 285
Application: Useful for cell culture and for the study of signaling pathways.
Biological Activity: Measured by its ability to stimulate human B lymphocyte cells. The ED50 of this effect is between 5 - 10 ng/ml
Description: Recombinant human soluble BAFF (GenBank Accession No. NM_006573) is a 17.0 kDa (a.a. 134-285) protein expressed in an E. coli expression system.
Endotoxin Level: < 1EU per µg of BAFF as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered solution containing 0.5X PBS, pH 7.5 + 0.5 mM DTT, containing no additives.
Genbank: NM_006573
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C.
Tags: none
Uniprot: Q9Y275
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Knight AK, et al. Clin. Immunol. 124 (2), 182-189 (2007)
2. Hever, A., et al. Proc. Natl. Acad. Sci. U.S.A. 104 (30), 12451-12456 (2007)
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