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Applications: Useful for cell culture and for the study of signaling pathways.
Biological Activity: Measured by its ability to stimulate proliferation of NIH3T3 cells. The ED50 of this effect is between 0.1 - 1 ng/ml
Description: Recombinant human soluble basic FGF (GenBank Accession No. NM_002006) is a 17.3 kDa (134 aa-288 aa) protein expressed in an E. coli expression system.
Endotoxin Level: < 1EU per ug of bFGF as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered solution containing 5 mM Tris, pH 7.5, 150 mM NaCl
Genbank: NM_002006
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C.
Tags: none
Uniprot: P09038
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Matsui, Y., et al. Cell 70(5), 841-847 (1992).
2. Segev, H, et al. Stem Cells. 22(3), 265-74 (2002).
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