Conjugate: Biotin-SP (long spacer)
Physical State: Freeze-dried solid
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Specificity: IgG, F(ab')2 fragment
Information: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of chicken IgY. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against the Fc portion of chicken IgY or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
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