Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-321 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: BirA (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
Description: Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol.
Genbank: NP_418404
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: H0QFJ5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.