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Encompassing Amino Acids: 2-321 (end)
Applications: Useful for covalent in vitro biotin-labelingof receptor proteins fordetection and purification assays.
Description: Biotin ligase BirA, GenBank Accession No. AP012306, a.a. 2 - 321 (end) with N-terminal His-FLAG tag, MW = 37 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 80 mM imidazole.
Genbank: AP012306
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: H0QFJ5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.
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