Application Note: Bovine Collagen Type I purified protein standard is tested by SDS-PAGE and used as a control for SDS-PAGE, Western Blot, ELISA, immunoprecipitation, and for other immunological assays. Specific conditions should be optimized by user. Collagen type I is recognized by type specific Anti-Collagen antibodies that recognize a native three-dimensional structure.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:10,000
Immunohistochemistry Dilution: 1:100-1:500
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: 1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Bovine Collagen Type 1 has been prepared from Bovine Placenta and is chromatographically and immunologically pure. Bovine Collagen Type 1 reacts with anti-Collagen Type I. Reaction with Rockland’s anti-Collagen II, III, IV, V or VI is negligible.
Background: Bovine Collagen Type I is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body's total protein). Over two dozen types of collagen have been described; Type I is the most abundant form in the body. Collagen Type I is found in scar tissue, tendons, the skin, arterial walls, the corneas, muscles, cartilage, and in the organic parts of bones and teeth. Bovine Collagen Type I is ideal for investigators involved in extracellular matrix proteins and osteoporosis.
Low Endotoxin: No
Other: User Optimized