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Encompassing Amino Acids: 417-766 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA)containing 2 µg inactive MEK1 (BPS #40075), 5 µM ATP, and 0.1 - 1.6 ng/µl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.
Description: Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_004333
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P15056
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.
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