Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 432-725
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 350 mM imidazole.
Genbank: NM_004333
Host Cell Line: E. coli
Storage Stability: At least 12 months from date of receipt at -80⁰C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal His-tag (BRAF)
Uniprot: P15056
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.
English
Deutsch
