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Encompassing Amino Acids: BRAF 432-725, p50 2-378(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Genbank: NM_004333
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag (BRAF), N-terminal FLAG-tag (p50)
Uniprot: P15056
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.
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