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Encompassing Amino Acids: 306-417
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme was assayed according to BPS BRD3 (BD2) Assay Kit (Cat# 32523) protocol. BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen®(R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.
Description: Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 306 - 417 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 40 kDa, expressed in an E. coli expression system
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT
Genbank: NM_007371
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q15059
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.
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