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Encompassing Amino Acids: 49-170
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: BRD4 is incubated with biotinylated acetyl-histone peptide substrate (BPS# 33000) in BRD assay buffer (BPS# 33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® Nickel Chelate Acceptor Beads (PerkinElmer) are added, followed by AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.
Description: Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-170 corresponding to bromodomain 1 with N-terminal His-tag, MW = 15.5 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 1.28 µM imidazole, and 20% glycerol
Genbank: NM_058243
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: O60885
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov29;12(11):133.
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