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Encompassing Amino Acids: 22-138
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: BRDT protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.
Description: Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 22 - 138 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0,138 mM NaCl, 2.7 mM KCl, 1 mM EDTA,10% glycerol and 1 mM DTT
Genbank: NM_207189
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q58F21
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Berkovits, B.D., et al., Dev. Biol. 2011 Dec 15; 360(2): 358-368
2. Shang, E., et al., Development. 2007 Oct; 134(19): 3507-3515
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