Target: Brevican
Conjugate: RPE
Product Type: Monoclonal
Clone Number: N294A/6 (Formerly sold as S294A-6)
Immunogen: Fusion protein amino acids 219-655 of rat Brevican
Swiss-Prot: P55068
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~140kDa (and smaller due to proteolytic cleavage).
Cellular Localization: Extracellular Space,Extracellular Matrix,Membrane,Lipid-Anchor,GPI-Anchor
Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
References: 1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126.2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M. and Yamaguchi Y. (1995) Biochem. Biophys. Res. Commun. 216: 957-963.3. Rauch U., et al. (1997) Genomics 44: 15-21.4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and Hockfield S. (1998) J. Neurosci. 7: 2370-2376.5. Aspberg A., Adam S., Kostka G., Timpl R. and Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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