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Encompassing Amino Acids: 576-701
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed using the BPS Bioscience BRPF3 Inhibitor Screening Assay Kit (Catalog #32608) following the manufacturer's instructions.
Description: Human Bromodomain and PHD finger containing 3, or BRPF3, GenBank Accession No. NM_015695, a.a. 576 - 701 corresponding to single bromodomain with N-terminal GST-tag, MW = 41.2 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_015695
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9ULD4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ullah, M., et al., Mol Cell Biol. 2008 Nov;28(22):6828-6843
2. Mishima, Y., et al., Blood. 2011 Sep 1;118(9):2443-53
3. Slama, P., et al., Nucleic Acids Res. 2011 Mar;39(5):1666-1679
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