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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (Ser/Thr 21, Invitrogen) at room temperature for 1 hour.
Description: Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His-tag, MW=75 kDa, expressed in expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, and 10% glycerol.
Genbank: NM_003957
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q8IWQ3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bright N.J. et al., J. Biol. Chem. 283 (22), 14946-14954 (2008).
2. Guo Z. et al., Biochem. Biophys. Res. Commun. 347 (4), 867-871 (2006).
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