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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: Bruton's tyrosine kinase (BTK), is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. However, as therapies have progressed, specifically in chronic lymphocytic leukemia (CLL), resistance has become more common in patients where the disease has progressed due to prolonged therapy. A mutation in the BTK 481 cysteine residue to which inhibitors bind covalently is often linked to BTK inhibition (BTKi) in CLL patients where resistance has been led to disease progression. Targeting the BTK mutation of interest (C481S) has shown promising clinical efficacy in treating CLL patients that are no longer responding to common therapy.
Description: The BTK (C481S) Kinase Assay Kit is designed to measure BTK (C481S) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant BTK (C481S) kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Uniprot: Q06187
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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