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Encompassing Amino Acids: 578-872
Applications: Useful for the study of enzyme kinetics and for inhibitor screening.
Assay Conditions: Assay was done in kinasebuffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µMATP. Reaction was done at 30°C for 45 min.Amount of ATP transferred was calculatedusing Kinase-Glo reagent (Promega).
Background: c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family (1). The c-MER gene encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. Functional knockout of c-MER in mice shows the mice to have macrophages deficient in the clearance of apoptotic thymocytes thereby demonstrating the critical role for c-MER in the engulfment and efficient clearance of apoptotic cells. Defects in c-MER have been associated with retinitis pigmentosa, a phenotype that can be reversed by replacement of the c-MER gene product (2).
Description: Human c-Mer, also known astyrosine-protein kinase Mer, MERTK, andreceptor tyrosine kinase MerTK GenBankAccession NM_006343, a.a. 578-872 with CterminalGST-tag. Expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 60 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione
Genbank: NM_006343
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q12866
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Van Hoof, C. et al., Genomics. 1995; 28:261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23:535-546.
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