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Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: c-Met, also known as HGFR (hepatocyte growth factor receptor), is a receptor protein tyrosine kinase encoded by the gene MET. Upon binding its ligand HGF (hepatocyte growth factor), c-Met activates multiple cellular processes including proliferation, adhesion and angiogenesis. Importantly, many studies report that c-Met is overexpressed in various carcinomas, suggesting that targeting HGF/c-Met signaling could be a promising target for cancer treatment. A splice mutation that results in skipping exon 14 has been identified in ~4% of lung cancer patients, particularly those with non small cell lung cancer. This mutation causes over-expression of MET protein and increased MET activation, leading to oncogenesis.
Description: The c-Met (del 963-1009) Kinase Assay Kit is designed to measure the kinase activity of c-Met (del 963-1009, exon 14 deletion) for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The recombinant protein used in the kit corresponds to amino acids 956-1390 of c-Met that contain the tyrosine kinase domain. The recombinant protein also contains a deletion of amino acids 963-1009 corresponding to exon 14 skipping.
Storage Stability: Up to 6 months when stored as recommended.
Supplied As: The c-Met Del 14 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Met 14 Del enzyme, c-Met substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Awad, M.M., et al. "MET exon 14 mutations in non-small-cell lung cancer are associated with advanced age and stage-dependent MET genomic amplification and c-Met overexpression." J. Clin. Oncology (2016). 34 (7): 721-30
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