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Encompassing Amino Acids: 306-648
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µM ATP, 1 µM MEK1(K97R), 1 hour. ATP reduction was detected using ADP-Glo™ Kinase Assay Platform (Promega).
Description: Human RAF1, also known as c-RAF (GenBank Accession No. NM_002880), a.a. 306-648, containing the mutations Y340D and Y341D, with N-terminal GST-tag, MW=65 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT.
Genbank: NM_002880
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P04049
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lamberti,A. et al. Cell Death Differ. 14 (5), 952-962 (2007).
2. Yu,F. et al. PLoS Pathog. 3 (3), E44 (2007).
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