Target: Calnexin
Conjugate: ATTO 390
Product Type: Polyclonal
Immunogen: A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Swiss-Prot: P24643
Purification: Rabbit Antiserum
Storage Buffer: Rabbit Antiserum *Storage buffer may change when conjugated
Specificity: Detects the N-terminal domain of Calnexin ~90kDa.
Cellular Localization: Endoplasmic Reticulum,Endoplasmic Reticulum Membrane,Melanosome
Scientific Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
References: 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229.3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819.5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103.7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649.9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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