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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: CAMKIγ (CLICK-III), a member of CAMK family, is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. Full activation of CaMKIγ requires both Ca(2+)/CaM and phosphorylation by CAMKK. CAMKIγ transcripts is most abundant in neurons, with the highest levels in limited nuclei such as the central nucleus of the amygdala (CeA) and the ventromedial hypothalamus (1).
Description: Recombinant human CAMK1γ, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_020439
Host Cell Line: Sf9 cells
Purity: ≥85%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96NX5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Takemoto-Kimura,S. et al. J. Biol. Chem. 278 (20), 18597-18605 (2003).
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