Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: full length
Applications: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Varying amounts of enzyme were incubated in 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 4 mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA, 0.4 mM EDTA, 0.01 mM DTT, 0.05 mM ATP, 7.5 µg of Autocamtide 2 synthetic peptide substrate, Ca2+/Calmodulin solution, and 32 µCi of [33P]-ATP was incubated at 30°C for 15 minutes. Then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Description: Recombinant humancalcium/calmodulin-dependent proteinkinase 2 delta (CAMK2), also known asCAMKII, CAMK2d and CAMKIId,GenBank Accession No. NM_172115, fulllengthwith an N-terminal GST tag andexpressed in a baculovirus-infected Sf9insect cell expression system. MW = 77kDa.
Format: Aqueous buffer solution.
Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA,0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Genbank: NM_172115
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST tag
Uniprot: Q4G1A8
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Hoch, B., et al., Circ. Res. 1999; 84: 713- 721.
2. Zhu, W.-Z., et al., J. Clin. Invest. 2003; 111: 617-625.
English
Deutsch
