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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: CAMKK2 (CAMKKβ) is a member of the CAMKK family. It is broadly distributed in tissues with highest levels in brain, thymus, spleen, and testis (1). CaMKK2 undergoes intramolecular autophosphorylation, is regulated by Ca2+/calmodulin and phosphorylates CaMKI and CaMKIV on Thr177 and Thr200, respectively. CaMKK2 activates both CaMKI and CaMKIV when coexpressed in Jurkat T cells. CaMKK2 also phosphorylates and regulates the activity of AMPK, which is an important regulator of cellular metabolism in response to metabolic stress (2).
Description: Recombinant human CAMKK2, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_172226
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96RR4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Anderson, K.A. et al: Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta.
2. Hurley, R.L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6.
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