Abbreviation: cAMP
Target Synonym: Cyclic adenosine monophosphate;cAMP;cyclic AMP;3'-5'-cyclic adenosine monophosphate;
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cAMP. During the reaction, Universal cAMP in the sample or standard competes with a fixed amount of Universal cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal cAMP in samples.No significant cross-reactivity or interference between Universal cAMP and analogues was observed
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