Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 µg CA2. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.
Description: Human carbonic anhydrase II, (CA2), GenBank Accession No. NM_000067, a.a. 1-260(full length), with C-terminal His-tag, expressed in an E. coli cell expression system. MW = 30 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 106 mM imidazole and 20% glycerol
Genbank: NM_000067
Storage Stability: At least 6 months at -80°C
Tags: C-terminal His-tag
Uniprot: P00918
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Loiselle, F.B., et al., Am J Physiol Cell Physiol. 2004 Jun;286(6):C1423-33
2. Shah, G.N., et al., Hum Mutat. 2004 Sep;24(3):272
English
Deutsch
