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Encompassing Amino Acids: CD33 1-16, CathepsinF 20-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme activity wasmeasured in a buffer containing 100 mMSodium acetate (pH 5.5), 2 mM EDTA, 8 mMDTT, 0.05% Brij, and 20 µM substrate (Z-FRAMC),with 3.75-30 ng/µl Cathepsin F.Reaction was done at 30°C for 40-120min, and fluorescence signal was measuredat Ex360 nm/Em460 nm. Enzyme waspreactivated in 50 mM sodium acetate pH4.5, 5 mM DTT buffer for 90 min at RT prior toreaction.
Description: Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(a.a. 1-16) + cathepsin F (a.a. 20-end), with C-terminal His-tag, MW = 53 kDa (more than 58kDa after post-modification), expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 45 mM Na Acetate pH 5.5, 135 mM NaCl and 10% glycerol
Genbank: NM_003793
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q9UBX1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaakinen R. et al., Atherosclerosis 192 (2), 323-327 (2007).
2. Vazquez-Ortiz G. et al., BMC Cancer 5, 68 (2005).Application Reference(s):1. Orbe, J., et al. J Med Chem . 2015 Apr 9;58(7):2941-57. doi: 10.1021/jm501939z. Discovery and safety profiling of a potent preclinical candidate, (4-[4-[[(3R)-3-(hydroxycarbamoyl)-8-azaspiro[4.5]decan-3-yl]sulfonyl]phenoxy]-N-methylbenzamide) (CM-352), for the prevention and treatment of hemorrhage (2015)
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