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Encompassing Amino Acids: 18-333
Applications: Useful for activation of DPP1 (Cathepsin C). Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Z-Leu-Arg-AMC (20 µM)was used as a substrate. Reaction wasperformed in a buffer containing 50 mM MES(pH 5.0), 5 mM DTT for 21 min at RT.
Description: Human cathepsin L (GenBank Accession No. NM_001912), a.a. 18-333, with C-terminal His-tag, MW=36 kDa, expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_001912
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P07711
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jean D. et al., Biochem. J. 413 (1), 125-134 (2008).
2. Abboud-Jarrous G. et al., J. Biol. Chem. 283 (26), 18167-18176 (2008).
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