Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: CD33 1-16, CathepsinS 17-331
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM Sodium acetate pH4.5, 25 mM NaCl, 5 mM DTT, 10 µM substrate(Mca-R-P-K-P-V-E-Nval-W-R-K(Dnp)-NH2), 10min. at room temperature. Enzyme waspreactivated in 50 mM sodium acetate, pH4.5, 0.25 M NaCl, 5 mM DTT buffer for 2 hr. atroom temperature prior to reaction.
Description: Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(a.a. 1-16) + cathepsin S (a.a. 17-331), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 45 mM Tris7HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, 3 mM DTT, and 10% glycerol
Genbank: NM_004079.3
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P25774
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Seo H.R. et al., Int. J. Cancer 124 (8),1794-1801 (2009).
2. Lutzner N. and Kalbacher H. J. Biol.Chem. 283 (52), 36185-36194 (2008).
English
Deutsch
