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Encompassing Amino Acids: CD33 1-16, CathepsinV 18-334
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay is conducted in 25 mM MES buffer pH5.0, 0.1 M NaCl, 5 mM DTT and 20 μM substrate (Z-LeuArg-AMC) at room temperature for 30 minutes. Fluorescence intensity is measured at exc360/em460.
Description: Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(a.a. 1-16) + cathepsin V (a.a. 18-334), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 40 mM MES pH 5.0, 120 mM NaCl and 20% glycerol.
Genbank: NM_001333.2
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: O60911
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Puzer L. et al., Biochem. Biol. Chem. 389(2), 195-200 (2008).
2. Burden R.E. et al., Biol. Chem. 388 (5), 541-545 (2007).
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