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Applications: This cell line is useful for CD137 binding assays or downstream CD137-dependent signaling events.
Background: Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.
Description: Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.
Format: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Genbank: NM_003811
Host Cell Line: HEK293
Mycoplasma Testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. McNamara II, J.O., et.al. (2008) Multivalent 4-1BB binding aptamers co-stimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118: 376-386.2. Ma, B.Y., et.al. (2005) The expression and regulatory role of OX40 and 4-1BB heterodimers in activated human T cells. Blood. 106: 2002-2010.
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