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Applications: This kit is useful for screening for inhibitors of CD137 binding to CD137L.
Background: CD137, also known as 4-1BB, is a prototype co-stimulatory moleculeexpressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows arole for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway mayprovide a therapeutic avenue to treat autoimmune and inflammatory diseases.
Description: The CD137:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CD137 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Drenkard, D., et al., FASEB J. 2007; 21: 456-463
2. Kwon, B., et al., Immune Netw. 2009; 9(3): 84-89.
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