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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed using the CD137 [Biotinylated]: CD137L Inhibitor Screening Assay Kit (BPS Bioscience #72025) with CD137 [Biotin] enzyme titrated from 40 ng/rxn - 0 ng/rxn.
Description: Human CD137L, also known as 4-1BB ligand (4-1BBL), tumor necrosis factor ligand superfamily member 9, TNFSF9. GenBank Accession No. NM_003811, a.a. 50-254 with an N-terminal His-Avi-tag, expressed in a HEK293 cell expression system. MW = 26 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_003811
Host Cell Line: HEK293
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-Avi-tag
Uniprot: P41273
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Langstein, J. et al., J. Immunol. 1998; 160(5): 2488-2494.
2. Salih, H.R., et al., 2000; J. Immunol. 165(5): 2903-2910.
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