CD14 Positive Cell Isolation Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Isolate CD14-expressing cells from a mixed population, such as monocytes in PBMCs.Positively selected cells may be used for downstream applications such as genomic analysis, expression assays, protein isolation, flow cytometry, or in vitro differentiation into dendritic cells and macrophages.

Background: Monocytes are cytokine-producing and phagocytic innate immune cells. These cells are of interest in the study of autoimmune diseases, cancer immunotherapy, inflammatory disorders, and other fields. CD14 is a marker of cells from the myelomonocyte lineage, including monocytes and macrophages. In PBMCs derived from healthy individuals, about 10-20% of the cells are CD14⁺ cells.

Description: The CD14 Positive Cell Isolation Kit is designed to magnetically separate CD14-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD14 positive cells from human peripheral blood mononuclear cells (PBMCs). Cells are incubated with an antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, your CD14 positive cells will be immobilized along the side of the tube while undesired CD14-negative cells will remain in suspension and can be easily removed.

Storage Stability: This cell isolation kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Supplied As: This kit provides enough reagents and materials for isolation of CD14+ cells from up to 1x10⁸ or 1x10⁹ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.

Uniprot: P08571

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• SKU: BPS78897-2
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78897-2
• Package Unit: 1 x 10⁹ Cells
• Quantity Unit: STK