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Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A CD157 hydrolase protocol was followed: 5 µl of a 10% DMSO solution was pre-incubated with 40 µl of CD157 (BTS1), His-Tag Recombinant enzyme for 30 minutes at Room Temperature (RT) before addition of 5 µl of 600 µM E-NAD substrate. The reaction was incubated for 1 hour at RT before fluorescence intensity was measured (λex = 300 nm; λem = 410 nm).
Background: CD157, also known as BST1 (bone marrow stromal cell antigen-1) is a glycosylphosphatidylinositol (GPI)-anchored molecule
with the dual functions of receptor and β-NAD+ metabolizing ectoenzyme, of the ADP-ribosyl cyclase family. This protein is expressed in the myeolomonocytic lineage (monocytes, neutrophils and others) and mesenchymal stem cells, mesothelial cells and many others. It plays a role in leukocyte adhesion, migration and diapedesis via its heparin-binding domains. Abnormalities in this protein are implicated in inflammatory disorders, such as RA (rheumatoid arthritis). A potential therapeutic avenue for disorders where CD157 is implicated is the disruption of its interaction with ECM (extracellular matrix) proteins.
Description: Recombinant human CD157, also known as BST1 (bone marrow stromal cell antigen 1), encompassing amino acids 29-292. This construct contains a C-terminal His-tag (6xHis). The protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-Tag
Uniprot: Q10588
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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