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Applications: Useful for studying protein binding and screening small molecules.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Casein substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent (Promega).
Description: Human secreted CD160, also known as Natural killer cell receptor BY55, GenBank Accession No. NM_007053, a.a. 27-159 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 15.6 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_007053
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Tags: C-Terminal His-tag
Uniprot: O95971
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cai, G., et al., Nat. Immunol. 2008; 9: 176-185.2. Le Bouteiller, P. et al., Proc. Nat. Acad. Sci. USA 2002; 99(26): 16963- 16968.
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