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Applications: This kit is useful for screening for inhibitors of CD70 binding to CD27.
Background: The tumor necrosis factor (TNF) receptor family member CD27 (TNFRSF7) and its TNF-like ligand CD70 (CD27L, TNFSF7) are a key costimulatory immune checkpoint signal for lymphocyte activation. Among other effects, this interaction promotes the differentiation of T and B cells and participates in immunoglobulin synthesis. CD27:CD70 interaction has been demonstrated to have important roles in autoimmunity, inflammation, infection and cancer, and antibodies targetingeither CD27 or CD70 are in clinical trials for oncology.
Description: The CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD27:CD70 signaling. This kit comes in a convenient 96- well format, with biotin-labeled CD70, purified CD27, streptavidin-labeled HRP, and assay buffer for100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD70by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD27 is coated on a 96-well plate. Next, CD70 is incubated with CD27 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Granger, S.W., et al. Cytokine Growth Factor Rev. 2003, 14(3-4): 289-296
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