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Background: The activation of naive T cells requires two signals; the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-2 (CD86) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-2 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.
Description: The CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-2 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-2 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Genbank: NM_021893
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-2, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Tags: FC Fusion, PE Labeled
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143.2.Yao, S., et al. Immunity 2011, 34(5):729-40.
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