Clone: 33-2A3/GRM1+B-A19/D3/9.
Conjugate: FITC/PE+PE/PerCP.
The CD3 antibody recognizes 22/26/30 kD MW lymphocyte surface molecules associated with the T cell antigen receptor complex. Reacts with 85% of peripheral blood T lymphocytes, 70% of thymocytes, the majority of T cell chronic lymphocytic leukaemias, Sezary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin. The monoclonal antibody is directed against the CD16- antigen (the Fc gamma receptor III), which is expressed on granulocytes, NK lymphocytes and macrophages. The mobility of the CD16-antigen is dependent on the NA1/NA2 allotype of the neutrophil donor. The monoclonal antibody inhibits the binding of human IgG to the Fc gamma receptor III.The CD56 antigen is a 175-185kDa isoform of the neural cell adhesion molecule (NCAM). It is expressed by lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors. It is expressed on natural killer (NK) Cells and a subpopulation of T lymphocytes. NK cells make up approximately 10-25% of peripheral blood lymphocytes. The CD45 antibody recognizes 180, 195, 205, 220, kD MW components of the leucocyte common antigen complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen.
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