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Encompassing Amino Acids: 19-379
Application: Useful as a positive control for Western blotting
Description: Human CD30, Fc fusion protein, with C-terminal Avi-Tag, Biotin-Labeled, also known as Tumor Necrosis Factor Receptor Superfamily Member 8, and TNFRSF8, GenBank Accession No. NM_001243.4, a.a. 19-379 expressed in a HEK293 cell expression system. MW = 67 kDa. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001243.4
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Fc fusion Avi-Tag, Biotin-Labeled
Uniprot: P28908
Biosafety Level: Not applicable (BSL-1)
References: 1.Piccinni, M. et al., Journal of Immunology. 1995; 1: 128-133.2.Smith, C.A. et al. Cell. 1993; 73(7): 1349-1360.
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