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Encompassing Amino Acids: 21-193
Application: Useful for Western blots, studying protein binding, and for screening small molecules and antibodies.
Assay Conditions: Reaction done according to the protocol for the CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit, #79257). Plate was coated with 100 ng/well CD40L (#71191) at 4°C overnight. Various concentrations of biotinylated CD40 (0-1 µM) added for 2h at RT. Strep-HRP added for 1h at RT.
Description: Human CD40, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, Bp50. GenBank Accession No. NM_001250, a.a. 21-193, fused at the C-terminus with the Fc portion of Human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW = 48 kDa. This protein runs at a higher molecular weight due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001250
Storage Stability: At least 6 months at -80°C.
Tags: Fc (IgG1), Avi-Tag, Biotin Labeled
Uniprot: P25942
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lutgens, E. et al., Nat. Med. 1998; 5: 1313-1316.2. Quezada, S.A., et al., Immunology. 2003; 22: 307-328
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