Clone Name: 5C8
Light Chain: Kappa
Product Description: The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.
Application Note: FACS: 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 29
Form: Liquid
Buffer (with preservative): 10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin, 0.09% Sodium azide.
Concentration: 0.05 mg/ml (Please refer to the vial label for the specific concentration.)
Background: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Uniprot ID: P29965
Purification: Purified by affinity chromatography
Conjugation: APC
Full Name: CD40 ligand
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