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Applications: Screen for antibodies binding to CD43 or examine CD43 function in a cellular context
Background: CD43 is a heavily glycosylated type I transmembrane protein expressed on the surface of most hemopoietic cells. It is abundantly expressed on the surface of T cells and functions as a co-stimulatory molecule by transducing activation signals through its cytoplasmic domain. Ultimately, it is thought that CD43 contributes to downstream T cell gene regulation and modulates cell function.
Description: Recombinant CHO cell line stably expressing full length human CD43 gene (sialophorin, gpL115, leukosialin, SPN; ref. seq. NM_003123) under the control of the CMV promoter.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/StreptomycinGrowth Medium 3D (BPS Bioscience #79539):
F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin
Genbank: NM_003123
Host Cell Line: CHO
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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