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Encompassing Amino Acids: 632-1304
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 20 mM HEPES, pH 7.2,100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1mM PNPP, 20 min at room temperature.Absorbance was measured at 405 nm.Specific activity was calculated based onpnp=18,000M-1cm-1.
Background: PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
Description: Protein tyrosine phosphatase CD45, also known as PTPRC (GenBank accession No. NM_080922), catalytic domain, a.a. 632-1304 (end) with N-terminal His-tag, MW=81 kDa, expressed in a Baculovirus Sf9 expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Genbank: NM_002838
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: P08575
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rachmilewitz, J. et al., J. Biol. Chem. 278, 14059-14065.
2. Fernandis, A.Z. et al., J. Biol. Chem. 278, 9536-9543.
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